Research

Crystallization is a major issue in the development (purity, bioavailability ...), the production (feasibility, yield ...) and the industrial property (process patents, polymorphs ...) of fine chemicals. Efficient achieving of crystallization objectives requires specific knowledge, tools and skills.

Holodiag provides crystallization process development and optimization services. The Holodiag experts master the fields of:

  • Crystallization of small organic molecules (nucleation, growth ...)
  • Thermoynamics of heterogeneous equilibria
  • Phase diagrams
  • Crystallography
  • Molecular modelling


Holodiag owns a laboratory dedicated to crystallization and phase characterization. The analytical devices (c.f. Analytical services for further description) ensure an adapted answer to problematics regarding polymorphism (post-screening) and enantiopurification / optical resolution.


pucePolymorphism

The main solid states encountered are:

    mandelic acid
  • Polymorphs: same composition but a different crystal packing
  • Hydrates/solvates (pseudo-polymorphs): a solvent is involved in the crystal packing
  • Salts: salification of an acid or a base giving a different crystal packing
  • Amorphous: non-crystalline solid exhibiting interesting bio-availability properties

The different possible solid states are highlighted by a preliminary screening step. However, the experimental crystallization conditions are not thoroughly investigated. Therefore, a post-screening step is needed to set up the parameters for the selective crystallization of a given solid state.

Holodiag provides R&D services for post-screening studies. Heterogeneous equilibria and phase diagrams (monotropy, enantiotropy, peritectic, non-congruent solubility ...) allow the determination of optimized crystallization conditions.



puceEnantiopurification / optical resolution

Access to pure enantiomers can be achieved by different methods. Crystallization is an efficient and cost-effective tool for the enrichment of mixtures or the enantiomeric resolution. Several routes can be contemplated:

    mandelic acid
  • Diastereomeric salts
  • Preferential Crystallization
  • Anti-conglomerate


Development and optimization are achieved by rationalization of the physico-chemical phenomena. For the determination of the best crystallization conditions with respect to the customer needs (objectives) only few tests are needed (no blind screening is performed to set up the conditions).